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Phasing with softer X-ray radiation in-house

AppNote PX008: SAD phasing with Cr X-ray radiation

Background

With the addition of Cr radiation to the crystallographer’s toolkit, in-house X-ray sources can routinely provide this softer wavelength as an option. This report discusses the results of SAD and anomalous scattering aided molecular replacement experiments with Cr radiation. We also report the results of data collection that allows the collection of data with Cr radiation from a single crystal in one diffraction experiment suitable for both phasing and refinement.

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